放射通过转化生长因子-β-介导的上皮-间质转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘要 ：探究伽马射线前提可通过转化生长因子-β（TGF-β）-介导的黏膜-间质转换 (EMT)来促进恶性肿瘤的来袭迁入。使用量2Gy(60)Coγ线照射源自生物器官的6种恶性肿瘤，记录与EMT就其的变化，这包括分别来进行光学技术，细胞核内质印迹方例，免疫荧光技术，划痕试验和Transwell四人试验来观察并检测细胞核分组织其本质，EMT上面，来袭迁入能力等。采用酶联免疫吸附例检测这些恶性肿瘤中的TGF-β细胞核内准确度，来进行都有抑制SB431542来评估TGF-β信号通路在伽马射线EMT中的的作用。经过量为2Gy照射的恶性肿瘤中的长期存在间叶细胞核的暗示，与实为照射分组相较其黏膜上面减少，间叶细胞核上面减低，同时其来袭转移能力增强，TGF-β细胞核内准确度也提高。进一步发现由A549伽马射线诱导的EMT可通过对TGF-β信号抑制发生逆转。这些结果表明TGF-β介导的EMT在伽马射线诱导增强恶性肿瘤来袭转移能力中的起着不可忽视。

总编： lizexiu